Browsing by Author "Benavides Pardo, Stefania Nicole"
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- ItemFrecuencia de linfadenitis en cuyes e identificación de los agentes bacterianos de la provincia de Cotopaxi.(Ecuador: Latacunga: Universidad Técnica de Cotopaxi (UTC)., 2024-08) Benavides Pardo, Stefania Nicole; Herrera Yunga, Vanessa del RosarioThe present research project aimed to determine the frequency of lymphadenitis and identify the causative agents in guinea pigs from the province of Cotopaxi through bacterial isolation and biochemical tests. To achieve this, 11 samples were processed from lymph nodes, liver, lung, kidney, bladder, and rectal swabs, conducting a microbiological isolation analysis according to the manual of the Spanish Society of Infectious Diseases and standardized international norms. The laboratory procedures employed were modified for both gram-positive and gram-negative protocols. Subsequently, biochemical tests were conducted for identification. The results showed a higher frequency of lymphadenitis in the Canton of Latacunga, with a percentage of 35% (6/11), and lower percentages in the cantons of Pujilí with 31% (2/11) and Saquisilí with 34% (3/11). The most affected organ concerning gram-positive bacteria was the intestine, with 50%, while for gram-negative bacteria, the lymph node was the most affected organ, with 46%. The percentage of predominant bacteria was 56% Staphylococcus, 57% Streptococcus, 40% Trueperella, and among gram-negative bacteria, 63% Salmonella spp., 100% Citrobacter spp., and 100% E. coli in the lung as the sole etiological agent in the sampled organ. In conclusion, lymphadenitis is associated with various primary and secondary bacterial pathogens, causing lesions in different organs, consequently increasing morbidity and mortality rates and complicating the discovery of effective treatments. Therefore, control and biosecurity measures must be implemented to prevent the spread of the disease.