Browsing by Author "Guato González, Karen Mishell"

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    Evaluación del tiempo de estabilización de crioconservación de semen equino utilizando dos diluyentes de origen animal en equinos del CEASA – Salache Bajo – Latacunga.
    (Ecuador: Latacunga: Universidad Técnica de Cotopaxi (UTC)., 2024-08) Guato González, Karen Mishell; Herrera Ariza, Kamila Mileysha; Beltrán Romero, Cristian Fernando
    The present research project was carried out with the objective of evaluating the stabilization time in the process of cryopreservation of equine semen using diluents of animal origin in Cotopaxi - Salache bajo, equine breeders lack strategies and specialized reproduction centers, due to complications in their management and high economic costs, generating limitations in efficiency and reproductive evaluation of stallions of high genetic value, cryopreservation increases the reproductive capacity of each stallion and allows for the efficient distribution of genetic material, this process reduces the spread of reproductive diseases. The development of the research was carried out at the Technical University of Cotopaxi and the control of two stallions; Moro (Lucitano) and Azim (Arabe), the type of collection was by artificial vagina three extractions at an interval of one week, the samples were analyzed macroscopically taking into account odor, pH, color, volume, and microscopically four main parameters were evaluated (stabilization time, cryopreservation, diluent, reproducer) compared in three times of 3, 6 and 9 hours, pre-freezing and post-freezing. The results obtained were measured by confidence intervals that have no significant difference between the four parameters evaluated, the evaluation by biplot that vigor has no relationship between individual motility and progressivity, the shorter the stabilization time, the greater the viability of the equine semen, avoiding parameters such as oxidative, thermal and osmotic stress that damage the sperm cell membrane, the equine with the highest sperm quality was the Lucitano breed, and an extraction protocol could be established for future research.