Browsing by Author "Intriago Moreira, Erika Karina"

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    Aplicación de la PCR para la detección de genes de virulencia en aislados de salmonella SPP. de cuyes del cantón Latacunga.
    (Ecuador: Latacunga: Universidad Técnica de Cotopaxi (UTC)., 2024-08) Intriago Moreira, Erika Karina; Herrera Yunga, Vanessa del Rosario
    Salmonellosis is a bacterial infection, what can begin with an outbreak and then, it stabilizes as an endemic disease, this involves, what the disease can appear sudden manner and then, it maintains constant form in the population. The guinea pig, like any species, is susceptible to infectious diseases, such as bacterial, viral, fungal and parasitic diseases. The microbiological studies lack, the economic losses main factor, which exists in the guinea pigs breeding. Salmonellosis is a bacterial disease, what has as etiological agent caused Salmonella sp., belonging to the Enterobacteriaceae family, the which are gram-negative bacilli, facultative anaerobes, what do not contain capsules or spores and are mobile by the peritrichous flagella presence. There are more than 2,500 different Salmonella serotypes that have been identified so far, they are mostly oxidase-negative, hydrogen sulphate producers, they have their average growth temperature ranges for their growth, the which from 5°C to 47°C, and with fewer temperatures to 7°C their growth stops. The research project had as aim PCR application for the virulence genes detection into Salmonella spp. isolates from Latacunga canton guinea pigs. Salmonella has virulence genes, which meet biological functions associated to the innkeeper recognition, adhesion and invasion, survival and macrophages death, among others, what enables the development and disease maintenance in guinea pigs and other species. By consequent, it was set out to determine the Salmonella spp virulence genes starting at 24 collected samples (rectal swabs, liver, abscesses and lung). The methodology consisted in the PCR application and it was used the following genes: spiA, sipB, tolC, sitC and sopB, which were revealed thanks to a 2% agarose gel to the horizontal electrophoresis chamber. The results reflected, what 99.2 % the genes, they were amplified with the respective base pair sizes. The Salmonella spp isolates indicated, what the virulence patterns are similar.