Browsing by Author "Jeréz Castro, Yajaira Katherine"

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    Prevalencia de reovirus en aves de traspatio en los cantones Salcedo y Pujilí en la provincia de Cotopaxi.
    (Ecuador: Latacunga: Universidad Técnica de Cotopaxi (UTC)., 2024-08) Jeréz Castro, Yajaira Katherine; Cueva Salazar, Nancy Margoth
    Reovirus disease (RNA) is one of the viral conditions with a low degree of pathogenesis because it is not a public health problem due to its zoonosis, it mainly affects the decrease in production, causing low mortality in the birds that acquire it. Reovirus affecting owners on a productive and economic level. The objective of this research was to determine the prevalence of Reovirus in backyard birds, the same one that developed in the rural area of the province of Cotopaxi, specifically in the parishes belonging to the Salcedo and Pujilí cantons that represent the largest geographical area, with the purpose to determine the prevalence of ANN in backyard birds through the use of the indirect enzyme-linked immunosorbent assay (ELISA-i) for the population detection of birds that present antibodies to the disease in the province of Cotopaxi. 165 backyard birds taken at random from rural parishes were used, what we tried to achieve with this was to find out how many animals had relatively positive titers to the disease, for which blood samples were taken from the birds, obtained directly from the brachial vein by direct puncture with a syringe and transported in red cap vacutainer tubes to the laboratory for the extraction of blood serum. The analysis used was the indirect ELISA technique, which consists of processing the sample through the enzymatic reaction by staining for subsequent analysis through the immune reaction of the pathogen to the reflection of light on the plate, reflecting relevant data in case of that the bird is affected by the disease. As results, it was found that according to the total variable they denoted a positive percentage in Salcedo with 71.24% while with 28.76% they were found negative. The same way in Pujili with a positive percentage of 86.96%, while 13.04% were negative.