Browsing by Author "Marcalla Constante, Diana Mireya"

Now showing 1 - 1 of 1
  • No Thumbnail Available
    Item
    Evaluación del efecto de dos diluyentes comerciales sobre el semen canino refrigerado en razas braquiocefálicas.
    (Ecuador: Latacunga: Universidad Técnia de Cotopaxi: (UTC), 2024-08) Marcalla Constante, Diana Mireya; Tulmo Segovia, Consuelo Marilin; Veloz Veloz, Dina Maricela
    Nowadays, the evaluation of effects of commercial diluents on canine semen refrigerated in brachiocephalic breeds such as the French bulldog, the Pekingese, Pug represents a crucial challenge in veterinary assisted reproduction. Specific anatomical data of these breeds, such as the facial structure characteristic that may predispose to respiratory and thermoregulatory problems highlight the need to optimize semen conservation techniques to improve sperm viability. Therefore, the objective of this research work is to evaluate the effect of two commercial diluents on canine refrigerated canine semen in brachiocephalic breeds. In the laboratory of Reproductive Biotechnology of the Technical University of Cotopaxi, 8 male randomly canines males of brachiocephalic and mixed dogs with ages ranging from 1 to 8 years old and in good health conditions were used. The seminal samples were extracted by manual collection technique, using the second fraction of the ejaculate, evaluated with the help of an electron microscope and a Neubauer chamber, the macroscopic characteristics such as volume, color, pH and microscopic characteristics were analyzed Pre-refrigeration and post-refrigeration evaluations were carried out, taking specific data with the two treatments that corresponded to a diluent of animal origin, to which animal protein such as egg yolk was added, and the second treatment corresponded to a diluent of vegetable origin. The data obtained during the experimental process were analyzed and processed with the help of the Excel program. For each of the parameters evaluated, it was found that there are significant differences in the percentage values between the use of diluent of animal origin, obtaining optimal results in the macroscopic and microscopic characteristics of the seminal material both in pre-assessment cooling and post-cooling, while the use of diluent from vegetal origin obtained low results in microscopic characteristics. These results show that the use of the seminal sample with the animal diluent produces better results during refrigeration that using the diluent of vegetable origin.